Marker Substance Library
Recommended Reading: 【Cheminformatics】 Substance Library
1. Overview
1. Overview
⑴ Additional missing information can be referenced from the curation of billions of papers at https://typeset.io/, https://www.scinapse.io/.
2. Molecular Pathways
○ annexin-V staining
○ TUNEL assay (terminal transferase dUTP nick-end labeling)
○ γ-H2AX assay
○ Calcein-AM assay
○ PI (propidium iodide assay)
○ Ki-67 assay
○ DAPI
○ CD8-PD1
○ caspase 3/7
○ PARP cleavage
○ 8-OHdG (8-hydroxy-2’-deoxyguanosine)
⑵ Other signaling molecules
○ Cdc42: A highly conserved GTPase regulating the polarity of many cells
3. Cellular Actions
⑴ Vesicle Activity Markers
○ clathrin-mediated endocytosis: transferrin
○ caveolae-mediated endocytosis: cholera toxin B subunit
○ macropinocytosis: 10 kDa dextran
○ endocytosis
○ stabilin-1
○ stabilin-2
○ adaptor protein (AP2M1)
○ caprin-1 (hypothetical)
○ SIDT2 (Systemic RNA interference deficient-1 transmembrane family 2) (hypothetical)
○ EGFR (epidermal growth factor receptor)
○ ESCRT-1 protein (e.g., TSG101 (tumor susceptibility gene 101), VPS28)
○ endosome maturation
○ annexin A2
○ EGFR (epidermal growth factor receptor)
○ PKCα (protein kinase C-alpha)
○ ESCRT-1 protein (e.g., TSG101 (tumor susceptibility gene 101), VPS28)
○ early endosome before endosomal escape: Rab5, APPL1, EEA1 (early endosome antigen1)
○ early endosome during endosomal escape: Rab5, EEA1, Rab11
○ recycling endosome: Rab11
○ non-productive arrested endosome: EEA1
○ endosomal escape
○ galectin-3
○ galectin-9: Superior to galectin-3
○ COPII (coat protein complex II) (e.g., SEC31a, Sar1, STX5)
○ GCC2
○ M6PR (mannose-6-phosphate receptor)
○ Alix
○ late endosome
○ CD63
○ Rab7
○ Rab9: Mediates transport to the trans-Golgi network
○ LBPA (lysobisphosphatidic acid)
○ LAMP1: lysosome-associated membrane protein 1
○ lysosome: LAMP1, Lysotracker (lysosome-specific dye)
○ autophagosome: LC3 (LC3B), LAMP1
⑵ Structural Markers
○ α-SMA (ACTA2, Acta2)
○ PanCK: Antibody cocktail mixture identifying various types of cytoskeleton
○ TESC: Bud tip progenitor marker
4. Physiological Actions
⑴ Immune System Markers
○ CD274, PDCD1: PD-L1/PD1-related markers
○ miRNA155: APC maturation marker
○ SIINFEKL: MHC class marker
○ H-2kb: MHC class I marker
○ MHC II: APC marker
Input: 2022.09.09 14:08