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Chapter 3-2. Relationship between enzyme activity and substrate

Higher category: 【Biology】 Chapter 3. Cell and Material Metabolism

Q.

Enzyme A loses its activity at 50℃ due to oxygen molecule, converting into an inactive form (D). The inactivation rate constant, k₁, is 0.002 / M·s. On the other hand, the enzyme reversibly undergoes a form change and partially unfolds into an unstable unfolded form (N’). It becomes more sensitive to oxygen and eventually loses its activity, and the inactive rate constant k₂ from the unfolded form is 0.3 / M·s. Meanwhile, the apparent inactivation rate constant (k_obs) of enzyme A with respect to oxygen at 50 ℃ was measured to be 0.05 / M·s.

⑴ What is the equilibrium constant between native form (N) and unfolded form (N’)?

⑵ What would be the inactivation rate constant if there was a reaction substrate of enzyme A in the above experiment?

① Fast equilibrium assumption

⑶ Explain the measures to improve the productivity of an enzyme.

① First, establish optimal pH, temperature, and ion gradients (e.g., chelate ions).

② Second, enhance the stability of the enzyme by adding substrates or substrate analogs.

Input: 2017.9.22 14:56